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Release of elements from municipal solid waste incineration fly ash

Wei WANG, Lei ZHENG, Feng WANG, Xiao WAN, Keqing YIN, Xingbao GAO

《环境科学与工程前沿（英文）》 2010年第4卷第4期页码 482-489 doi: 10.1007/s11783-010-0245-7

摘要： The element-release behavior of municipal solid waste incineration fly ash was explored through leaching test with continuous set-point pH (pH test) and serial single reaction cell (SSRC) tests. First, the relationship between element release and acid neutralizing capacity (ANC) consumption was examined with a pH test. Four types of release behaviors were identified which are characteristic for different elements: (1) release curves that were almost linear with ANC consumption (Ca, Zn, and Cd); (2) release that was significantly faster than ANC (Na, K, and Cl); (3) curves that featured a strong increase with ANC consumption, after a transient release, followed by an almost equal decrease (Si and S); and (4) release that is strongly retarded compared with ANC consumption (Cr, Cu, and Pb). In the SSRC system, it the existence of a pH front and a wash-out phenomenon is demonstrated. Combining the results from the SSRC test with the kinetic analysis of the ANC system in the pH test, it was inferred that less than one-third of the ANC measured from a batch pH titration plays a neutralization role in a field situation. The methodologies described may provide a powerful set of tools for systematic evaluation of element release from solid wastes.

关键词： pH_stat test Serial single reaction cell test (SSRC) leaching heavy metal

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Identify the contribution of vehicle non-exhaust emissions: a single particle aerosol mass spectrometertest case at typical road environment

《环境科学与工程前沿（英文）》 2023年第17卷第5期 doi: 10.1007/s11783-023-1662-8

摘要：

● A single particle observation was conducted in a high traffic flow road environment.

关键词： Non-exhaust emissions SPAMS PMF Roadside environment

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Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell

《医学前沿（英文）》 doi: 10.1007/s11684-023-1017-7

摘要： The association of neurogenesis and gliogenesis with glioma remains unclear. By conducting single-cell RNA-seq analyses on 26 gliomas, we reported their classification into primitive oligodendrocyte precursor cell (pri-OPC)-like and radial glia (RG)-like tumors and validated it in a public cohort and TCGA glioma. The RG-like tumors exhibited wild-type isocitrate dehydrogenase and tended to carry EGFR mutations, and the pri-OPC-like ones were prone to carrying TP53 mutations. Tumor subclones only in pri-OPC-like tumors showed substantially down-regulated MHC-I genes, suggesting their distinct immune evasion programs. Furthermore, the two subgroups appeared to extensively modulate glioma-infiltrating lymphocytes in distinct manners. Some specific genes not expressed in normal immune cells were found in glioma-infiltrating lymphocytes. For example, glial/glioma stem cell markers OLIG1/PTPRZ1 and B cell-specific receptors IGLC2/IGKC were expressed in pri-OPC-like and RG-like glioma-infiltrating lymphocytes, respectively. Their expression was positively correlated with those of immune checkpoint genes (e.g., LGALS3) and poor survivals as validated by the increased expression of LGALS3 upon IGKC overexpression in Jurkat cells. This finding indicated a potential inhibitory role in tumor-infiltrating lymphocytes and could provide a new way of cancer immune evasion.

关键词： single-cell RNA-seq glioma radial glia primitive oligodendrocyte precursor cell immune escape

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Numerical analysis of bearing behaviors of single batter piles under horizontal loads in various directions

《结构与土木工程前沿（英文）》 2023年第17卷第2期页码 224-237 doi: 10.1007/s11709-022-0914-1

摘要： The horizontal bearing behavior of a single batter pile (SBP) is vital to its application in practical engineering; however, the horizontal responses of SBPs change with the directions of horizontal loads, and this phenomenon is rarely investigated. Therefore, the directional differences in the horizontal bearing behaviors of SBPs are investigated in this study. Four model tests are conducted to preliminarily examine the effects of the skew angle of horizontal loads on the horizontal bearing capacities and distributions of the bending moments of the SBPs. Subsequently, the differences in the responses of the SBPs under horizontal loads in various directions at full scale are analyzed comprehensively via finite-element (FE) analysis. The effects of the skew angle on SBP-soil interaction are discussed. Moreover, an empirical design method is proposed based on the FE analysis results to predict the bearing ratios of SBPs in medium-dense and dense sand while considering the effects of the skew angle, batter angle, and pile diameter. The method is confirmed to be effective, as confirmed by the close agreement between the predicting results with the model test (reported in this study) and centrifuge model test results (reported in the literature).

关键词： single batter pile skew horizontal load model test finite-element analysis empirical design method

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Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in

《医学前沿（英文）》页码 939-956 doi: 10.1007/s11684-023-0987-9

摘要： A small proportion of mononuclear diploid cardiomyocytes (MNDCMs), with regeneration potential, could persist in adult mammalian heart. However, the heterogeneity of MNDCMs and changes during development remains to be illuminated. To this end, 12 645 cardiac cells were generated from embryonic day 17.5 and postnatal days 2 and 8 mice by single-cell RNA sequencing. Three cardiac developmental paths were identified: two switching to cardiomyocytes (CM) maturation with close CM–fibroblast (FB) communications and one maintaining MNDCM status with least CM–FB communications. Proliferative MNDCMs having interactions with macrophages and non-proliferative MNDCMs (non-pMNDCMs) with minimal cell–cell communications were identified in the third path. The non-pMNDCMs possessed distinct properties: the lowest mitochondrial metabolisms, the highest glycolysis, and high expression of Myl4 and Tnni1. Single-nucleus RNA sequencing and immunohistochemical staining further proved that the Myl4⁺Tnni1⁺ MNDCMs persisted in embryonic and adult hearts. These MNDCMs were mapped to the heart by integrating the spatial and single-cell transcriptomic data. In conclusion, a novel non-pMNDCM subpopulation with minimal cell–cell communications was unveiled, highlighting the importance of microenvironment contribution to CM fate during maturation. These findings could improve the understanding of MNDCM heterogeneity and cardiac development, thus providing new clues for approaches to effective cardiac regeneration.

关键词： mononuclear diploid cardiomyocytes cell–cell communication cardiac fibroblast single-cell RNA sequencing cardiac regeneration

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PathogenTrack and Yeskit: tools for identifying intracellular pathogens from single-cell RNA-sequencing

《医学前沿（英文）》 2022年第16卷第2期页码 251-262 doi: 10.1007/s11684-021-0915-9

摘要： Pathogenic microbes can induce cellular dysfunction, immune response, and cause infectious disease and other diseases including cancers. However, the cellular distributions of pathogens and their impact on host cells remain rarely explored due to the limited methods. Taking advantage of single-cell RNA-sequencing (scRNA-seq) analysis, we can assess the transcriptomic features at the single-cell level. Still, the tools used to interpret pathogens (such as viruses, bacteria, and fungi) at the single-cell level remain to be explored. Here, we introduced PathogenTrack, a python-based computational pipeline that uses unmapped scRNA-seq data to identify intracellular pathogens at the single-cell level. In addition, we established an R package named Yeskit to import, integrate, analyze, and interpret pathogen abundance and transcriptomic features in host cells. Robustness of these tools has been tested on various real and simulated scRNA-seq datasets. PathogenTrack is competitive to the state-of-the-art tools such as Viral-Track, and the first tools for identifying bacteria at the single-cell level. Using the raw data of bronchoalveolar lavage fluid samples (BALF) from COVID-19 patients in the SRA database, we found the SARS-CoV-2 virus exists in multiple cell types including epithelial cells and macrophages. SARS-CoV-2-positive neutrophils showed increased expression of genes related to type I interferon pathway and antigen presenting module. Additionally, we observed the Haemophilus parahaemolyticus in some macrophage and epithelial cells, indicating a co-infection of the bacterium in some severe cases of COVID-19. The PathogenTrack pipeline and the Yeskit package are publicly available at GitHub.

关键词： scRNA-seq intracellular pathogen microbe COVID-19 SARS-CoV-2

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Clinical characteristics and prognostic factors of patients with mature T-cell lymphoid malignancies:a single-institution study of 225 cases

null

《医学前沿（英文）》 2015年第9卷第4期页码 468-477 doi: 10.1007/s11684-015-0419-6

摘要：

Mature T-cell lymphoid malignancies comprise a group of heterogeneous diseases that vary in clinicopathological features, biological behavior, treatment response, and prognosis. Bone marrow (BM) infiltration is more commonly present in mature T-cell lymphoid malignancies compared with their B-cell counterparts and hence important for differential diagnosis. In this study, clinical characteristics and prognostic factors were analyzed in 225 patients with mature T-cell lymphoid malignancies treated in a single institution. These included 29 cases of T-cell lymphoproliferative disorders (T-LPD, all with BM infiltration) and 196 cases of T-/natural-killer-cell lymphoma (T/NKCL, 56 with BM infiltration and 140 without BM infiltration). The estimated 5-year overall survival (OS) rates of T-LPD and T/NKCL were 96.6% and 37.3%, respectively. T-LPD patients were less likely to exhibit poor performance status, advanced disease stage, presence of B symptoms, or abnormal level of serum β-2 microglobulin. With similar pathological characteristics, T/NKCL patients with BM infiltration showed significantly lower response rates and shorter OS than those without BM infiltration (P = 0.0264 and P<0.0001, respectively). Multivariate analysis indicated that poor performance status, advanced disease stage, elevated serum lactate dehydrogenase level, and BM involvement were independent unfavorable prognostic factors. The Glasgow Prognostic Score may be more efficient than the International Prognostic Index in predicting disease outcome in T/NKCL. In conclusion, clinical characteristics may be useful in more effectively stratifying patients with mature T-cell lymphoid malignancies.

关键词： mature T-cell lymphoid malignancies clonal T-cell population bone marrow infiltration prognostic factors

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A new method of studying collapsibility of loess

Yuanqing ZHU , Zhenghan CHEN ,

《结构与土木工程前沿（英文）》 2009年第3卷第3期页码 305-311 doi: 10.1007/s11709-009-0040-3

摘要： A new triaxial testing system that could control suction in wetting-induced collapsible tests was successfully developed to study the suction effects on wetting-induced collapsible deformation. The pedestal of the triaxial cell was made up of two parts, and the equipment not only could control suction but also could make water accessible to soil. A pressure/volume-controlled equipment was combined with the triaxial system to measure the water volume absorbed by samples accurately and to add pressure on water to filtrate into the sample. The apparatus could measure volume change precisely and keep the deviator stress unvaried, as well as measure the volume of water filtrating into the samples exactly. A triaxial collapsible testing procedure was described using the new apparatus for undisturbed collapsible loess with controlled suction. Furthermore, a series of double triaxial collapsible tests were conducted under different suctions and the same net cell pressure, and tests under different net cell pressures and the same suction were also done. It was indicated that the collapsible deformation increased with the increasing suction, and the effect of the net cell pressure on collapsible deformation was remarkable. The new triaxial apparatus was a useful facility to study the collapsible behavior of loess.

关键词： triaxial apparatus for collapsible soils controlled suction pressure/volume controlled equipment double triaxial collapsible test single triaxial collapsible test

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Evaluation of regenerative braking based on single-pedal control for electric vehicles

Wei LIU, Xintian LIU, Yansong WANG, Hongzhong QI

《机械工程前沿（英文）》 2020年第15卷第1期页码 166-179 doi: 10.1007/s11465-019-0546-x

摘要： More than 25% of vehicle kinetic energy can be recycled under urban driving cycles. A single-pedal control strategy for regenerative braking is proposed to further enhance energy efficiency. Acceleration and deceleration are controlled by a single pedal, which alleviates driving intensity and prompts energy recovery. Regenerative braking is theoretically analyzed based on the construction of the single-pedal system, vehicle braking dynamics, and energy conservation law. The single-pedal control strategy is developed by considering daily driving conditions, and a single-pedal simulation model is established. Typical driving cycles are simulated to verify the effectiveness of the single-pedal control strategy. A dynamometer test is conducted to confirm the validity of the simulation model. Results show that using the single-pedal control strategy for electric vehicles can effectively improve the energy recovery rate and extend the driving range under the premise of ensuring safety while braking. The study lays a technical foundation for the optimization of regenerative braking systems and development of single-pedal control systems, which are conducive to the promotion and popularization of electric vehicles.

关键词： electric vehicle single-pedal control regenerative braking co-simulation dynamometer test

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TiO supported IrO for anode reversal tolerance in proton exchange membrane fuel cell

《能源前沿（英文）》 2022年第16卷第5期页码 852-861 doi: 10.1007/s11708-021-0811-7

摘要： Fuel starvation can occur and cause damage to the cell when proton exchange membrane fuel cells operate under complex working conditions. In this case, carbon corrosion occurs. Oxygen evolution reaction (OER) catalysts can alleviate carbon corrosion by introducing water electrolysis at a lower potential at the anode in fuel shortage. The mixture of hydrogen oxidation reaction (HOR) and unsupported OER catalyst not only reduces the electrolysis efficiency, but also influences the initial performance of the fuel cell. Herein, Ti₄O₇ supported IrO_x is synthesized by utilizing the surfactant-assistant method and serves as reversal tolerant components in the anode. When the cell reverse time is less than 100 min, the cell voltage of the MEA added with IrO_x/Ti₄O₇ has almost no attenuation. Besides, the MEA has a longer reversal time (530 min) than IrO_x (75 min), showing an excellent reversal tolerance. The results of electron microscopy spectroscopy show that IrO_x particles have a good dispersity on the surface of Ti₄O₇ and IrO_x/Ti₄O₇ particles are uniformly dispersed on the anode catalytic layer. After the stability test, the Ti₄O₇ support has little decay, demonstrating a high electrochemical stability. IrO_x/Ti₄O₇ with a high dispersity has a great potential to the application on the reversal tolerance anode of the fuel cell.

关键词： proton exchange membrane fuel cell (PEMFC) fuel starvation cell reverse reversal tolerance anode oxygen evolution reaction

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Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed

Ying Huo, Peng Yuan, Qingyuan Qin, Zhiqiang Yan, Liying Yan, Ping Liu, Rong Li, Jie Yan, Jie Qiao

《医学前沿（英文）》 2021年第15卷第1期页码 144-154 doi: 10.1007/s11684-020-0792-7

摘要： Oocyte cryopreservation is widely used for clinical and social reasons. Previous studies have demonstrated that conventional slow-freezing cryopreservation procedures, but not storage time, can alter the gene expression profiles of frozen oocytes. Whether vitrification procedures and the related frozen storage durations have any effects on the transcriptomes of human metaphase II oocytes remain unknown. Four women (30–32 years old) who had undergone IVF treatment were recruited for this study. RNA-Seq profiles of 3 fresh oocytes and 13 surviving vitrified-thawed oocytes (3, 3, 4, and 3 oocytes were cryostored for 1, 2, 3, and 12 months) were analyzed at a single-cell resolution. A total of 1987 genes were differentially expressed in the 13 vitrified-thawed oocytes. However, no differentially expressed genes were found between any two groups among the 1-, 2-, 3-, and 12-month storage groups. Further analysis revealed that the aberrant genes in the vitrified oocytes were closely related to oogenesis and development. Our findings indicated that the effects of vitrification on the transcriptomes of mature human oocytes are induced by the procedure itself, suggesting that long-term cryostorage of human oocytes is safe.

关键词： human metaphase II oocyte vitrification cryostorage duration single-cell RNA-Seq lncRNA

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Accelerated life-time test of MEA durability under vehicle operating conditions in PEM fuel cell

Tian TIAN, Jianjun TANG, Wei GUO, Mu PAN

《能源前沿（英文）》 2017年第11卷第3期页码 326-333 doi: 10.1007/s11708-017-0489-z

摘要： In this paper, a novel accelerated test method was proposed to analyze the durability of MEA, considering the actual operation of the fuel cell vehicle. The proposed method includes 7 working conditions: open circuit voltage (OCV), idling, rated output, overload, idling-rated cycle, idling-overload cycle, and OCV-idling cycle. The experimental results indicate that the proposed method can effectively destroy the MEA in a short time (165 h). Moreover, the degradation mechanism of MEA was analyzed by measuring the polarization curve, CV, SEM and TEM. This paper may provide a new research direction for improving the durability of fuel cell.

关键词： polymer electrolyte membrane fuel cell accelerated life-time test load cycling test durability

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A cytoprotective graphene oxide-polyelectrolytes nanoshell for single-cell encapsulation

Luanying He, Yulin Chang, Junhao Zhu, Ying Bi, Wenlin An, Yiyang Dong, Jia-Hui Liu, Shihui Wang

《化学科学与工程前沿（英文）》 2021年第15卷第2期页码 410-420 doi: 10.1007/s11705-020-1950-9

摘要： Graphene oxide (GO) has been increasingly utilized in the fields of food, biomedicine, environment and other fields because of its benign biocompatible. We encapsulated two kinds of GO with different sizes on yeast cells with the assistance of polyelectrolytes poly (styrene sulfonic acid) sodium salt (PSS) and polyglutamic acid (PGA) (termed as Y@GO). The result does not show a significant difference between the properties of the two types of Y@GO (namely Y@GO1 and Y@GO2). The encapsulation layers are optimized as Yeast/PGA/PSS/PGA/GO/PGA/PSS based on the morphology, dispersity, colony-forming unit, and zeta potential. The encapsulation of GO increases the roughness of the yeast. It is proved that the Y@GO increases the survival time and enhance the activity of yeast cells. The GO shell improves the resistance of yeast cells against pH and salt stresses and extends the storage time of yeast cells.

关键词： GO yeast polyelectrolyte cytoprotection nanomaterials

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Anion-exchange membrane direct ethanol fuel cells: Status and perspective

T.S. Zhao, Y.S. Li, S.Y. Shen

《能源前沿（英文）》 2010年第4卷第4期页码 443-458 doi: 10.1007/s11708-010-0127-5

摘要： Direct ethanol fuel cells (DEFCs) are a promising carbon-neutral and sustainable power source for portable, mobile, and stationary applications. However, conventional DEFCs that use acid proton-exchange membranes (typically Nafion type) and platinum-based catalysts exhibit low performance (i.e., the state-of-the-art peak power density is 79.5 mW/cm at 90°C). Anion-exchange membrane (AEM) DEFCs that use low-cost AEM and non-platinum catalysts have recently been demonstrated to yield a much better performance (i.e., the state-of-the-art peak power density is 160 mW/cm at 80°C). This paper provides a comprehensive review of past research on the development of AEM DEFCs, including the aspects of catalysts, AEMs, and single-cell design and performance. Current and future research challenges are identified along with potential strategies to overcome them.

关键词： fuel cell direct ethanol fuel cells anion-exchange membrane ethanol oxidation reaction oxygen reduction reaction cell performance

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intravenous busulfan conditioning with post-transplantation cyclophosphamide for allogeneic peripheral stem celltransplantation for adult patients with lymphoid malignancies: a prospective single-arm phase II study

Ling Wang, Lining Wang, Xing Fan, Wei Tang, Jiong Hu

《医学前沿（英文）》 2021年第15卷第1期页码 108-115 doi: 10.1007/s11684-019-0730-8

摘要： Post-transplantation cyclophosphamide (PT-Cy) alone or in combination with other immunosuppressive drugs has emerged as a promising strategy in the setting of allogeneic hematopoietic stem cell transplantation. Improved survival rate was reported in lymphoid malignancies following PT-Cy strategy compared with myeloid disease in non-myeloablative bone marrow transplant setting. Thus, we aimed to evaluate the safety and efficacy of PT-Cy combined with cyclosporine as graft-versus-host disease (GVHD) prophylaxis after myeloablative conditioning and T cell-replete peripheral stem cell transplantation in lymphoid malignancies. This single-arm phase II clinical trial (NCT01435447) involving 31 adult patients was conducted from January 2013 to June 2018. The donor-type neutrophil engraftment rate was 100%, and the overall incidence of grade II to IV and grade III to IV acute GVHD was 39% and 24%, respectively. The cumulative incidence rates of chronic GVHD (35%), including moderate to severe forms (10%), were reduced compared with those of the historical group ( =0.03 and =0.04, respectively). With a median follow-up of 18 months, the estimated 2-year overall and event-free survival was 64.8% (95% confidence interval: 47.8%–86.7%) and 58.4% (95% CI: 41.9%–81.7%), respectively. The 2-year cumulative incidence rate of relapse was 19.5% (95% CI: 9.0%–35.8%), whereas the non-relapse mortality rate was 21.8% (95% CI: 11.3%–38.1%). These results demonstrated the feasibility of PT-Cy as GVHD prophylaxis in this clinical setting. This strategy could significantly reduce the incidence of chronic GVHD and its moderate to severe forms but not of acute GVHD and results in similar survival outcomes compared with the historical group. A prospective study with additional patients is warranted to confirm the role of PT-Cy in lymphoid malignancy.

关键词： post-transplantation cyclophosphamide allogeneic hematopoietic stem cell transplantation lymphoid malignancies

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标题作者时间类型操作

Release of elements from municipal solid waste incineration fly ash

Wei WANG, Lei ZHENG, Feng WANG, Xiao WAN, Keqing YIN, Xingbao GAO

期刊论文

Identify the contribution of vehicle non-exhaust emissions: a single particle aerosol mass spectrometertest case at typical road environment

期刊论文

Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell

期刊论文

Numerical analysis of bearing behaviors of single batter piles under horizontal loads in various directions

期刊论文

Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in

期刊论文

PathogenTrack and Yeskit: tools for identifying intracellular pathogens from single-cell RNA-sequencing

期刊论文

Clinical characteristics and prognostic factors of patients with mature T-cell lymphoid malignancies:a single-institution study of 225 cases

null

期刊论文

A new method of studying collapsibility of loess

Yuanqing ZHU , Zhenghan CHEN ,

期刊论文

Evaluation of regenerative braking based on single-pedal control for electric vehicles

Wei LIU, Xintian LIU, Yansong WANG, Hongzhong QI

期刊论文

TiO supported IrO for anode reversal tolerance in proton exchange membrane fuel cell

期刊论文

Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed

Ying Huo, Peng Yuan, Qingyuan Qin, Zhiqiang Yan, Liying Yan, Ping Liu, Rong Li, Jie Yan, Jie Qiao

期刊论文

Accelerated life-time test of MEA durability under vehicle operating conditions in PEM fuel cell

Tian TIAN, Jianjun TANG, Wei GUO, Mu PAN

期刊论文

A cytoprotective graphene oxide-polyelectrolytes nanoshell for single-cell encapsulation

Luanying He, Yulin Chang, Junhao Zhu, Ying Bi, Wenlin An, Yiyang Dong, Jia-Hui Liu, Shihui Wang

期刊论文

Anion-exchange membrane direct ethanol fuel cells: Status and perspective

T.S. Zhao, Y.S. Li, S.Y. Shen

期刊论文

Ling Wang, Lining Wang, Xing Fan, Wei Tang, Jiong Hu

期刊论文

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